Plant Preservative Mixture (PPM) 植物組培抗菌劑
——植物培養防腐劑(抗菌劑)/生物殺滅劑/農藥
關(guān)鍵詞:植物培養抗菌劑(plant culture preservative),農藥(biocide),抗真菌劑,抑制農桿菌生長(cháng);
作用機制:
PPM(Plant Preservative Mixture,植物組培抗菌劑)是用于植物細胞培養中的一種強效且廣譜的生物殺滅劑,由多種抗菌劑組合而成的混合物,能殺死植物組培培養基和污染組織內的細菌和真菌,作用機制在于能夠穿透細菌或真菌細胞壁,抑制檸檬酸循環(huán)和電子傳遞鏈等中心代謝循環(huán)中關(guān)鍵酶的生物活性,以及抑制培養基內單糖和氨基酸往細菌或者真菌細胞的運輸。取決于PPM劑量和污染程度,PPM可用作植物培養基內的生物殺滅劑組分。PPM還可用作生物靜力化合物起到預防效果。除此之外,PPM能有效抑制植物培養液體或半固體培養中通過(guò)空氣、水、人傳播的微生物污染以及內源性污染,但不會(huì )影響體外種子發(fā)芽、愈傷組織生長(cháng)以及根莖再生。
適用植物類(lèi)型:
PPM有效作用于大多數種子裸露植物(被子植物和裸子植物),但不適合用于蕨類(lèi),蘚類(lèi),藻類(lèi)和水生植物?!?span>PPM是一種非常有效的抗微生物劑以預防和去除組培污染,然而不能替代實(shí)驗室的無(wú)菌處理試劑,建議使用合適的空氣無(wú)菌處理系統?!?span>
產(chǎn)品優(yōu)勢【與傳統的植物組培抗微生物劑相比】:
1)適當濃度下不會(huì )影響大多數植物生長(cháng);2)極低濃度即可維持無(wú)菌的培養環(huán)境;3)可預處理新生種子或清洗組織;4)熱穩定性強,高壓滅菌非常穩定;5)比傳統抗生素價(jià)格更低廉,可作為組培培養基內的標準成分,并作為常規使用;6)靶向抑制多種酶活性,植株產(chǎn)生耐藥性的幾率非常低;
產(chǎn)品特性:
外觀(guān):清澈,透明至琥珀色溶液,pH 3.8
保存和穩定性:4°C,3年有效
使用特點(diǎn):1)PPM可在滅菌前加入培養基或者分液前直接加入滅菌培養基;
2)PPM可在壓力15 psi(1.05 kg/cm2),121°C滅菌20min;
3)PPM需要滅菌后(post-autoclave)后加入含蛋白質(zhì)的培養基內,之后將混合好的培養基倒入培養皿;
4)工作濃度的PPM(經(jīng)培養基稀釋?zhuān)┛墒覝胤€定存放高達1個(gè)月;
使用濃度參考:
產(chǎn)品訂購:更大包裝或產(chǎn)品技術(shù)問(wèn)題,請來(lái)電/在線(xiàn)咨詢(xún),電話(huà):021-54736159 。 網(wǎng)站:optimisticestates.com。
貨號 |
品 名 |
規格 |
單價(jià) |
貨期 |
MS0006-30ML |
Plant Preservative Mixture (PPM) 植物組培抗菌劑 |
30ml |
648 |
現貨 |
MS0006-100ML |
Plant Preservative Mixture (PPM) 植物組培抗菌劑 |
100ml |
1688 |
現貨 |
應用文獻:
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